FastQCFastQC Report
Sat 18 Mar 2023
SRR6792049_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792049_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87665911
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC891140.10165182678589857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGT1744950.0138.738632
ACGTGAT1868400.0138.66662
ACGCTTA1351650.0138.606952
CAAGCTA1474800.0138.141682
AGTCACT1231050.0138.084271
CAGATTC1839350.0137.988882
GTCACTA1227400.0137.759192
ACGTATC1062200.0137.703311
ATGCCTA907100.0137.242751
TACCGTG1810500.0135.961413
ACAAGCT1538700.0134.648351
AGTTAGC857200.0134.600232
ATAGCGA861300.0134.591261
TCACGCA1144600.0134.222522
ACCTTAC1107550.0133.799242
TAGCGAC871350.0133.721762
AACGTGA1903500.0133.453521
TGCCTAA916550.0133.39692
TACCGTC811300.0133.283523
AGAGTCA918250.0132.431231
Produced by FastQC (version 0.11.5)