FastQCFastQC Report
Sat 18 Mar 2023
SRR6792047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149032389
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG90047826.042164431786704No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG22403311.5032510818839522No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14476800.9713861595548872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA14001800.9395138931846554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA8660050.5810850955358435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGTGAGATGGCTCAGT7543870.5061899665313692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC3121530.2094531276687781No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG2697920.18102910502226466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTC2143060.1437982719313451No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGAGAGATGGCTCA1518400.10188389317170511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT80026800.076.457351
AGTGGTA79873350.076.380215
GTATCAA80639400.075.842739
GTGGTAT81109700.075.175476
TGGTATC81494900.074.836917
AGCAGTG81632850.074.7961962
GGTATCA81645150.074.7671668
CAGTGGT82078750.074.34384
GCAGTGG82833350.073.617973
TAGTACC652950.073.1249145
CCCTCTT6461850.058.344963145
CCTCTTC3856300.051.404232145
CGTGGGG700600.040.59492145
CAGGGGA1975500.028.59535145
CGTGGGA1955500.026.948856145
TGCGGAT291550.025.711712145
ACCCGAC4839700.025.62524860-64
CCGTAAC2345450.025.37393125-129
ATGGGGG9662050.025.08798825-29
CACCCGA4998650.024.8420260-64
Produced by FastQC (version 0.11.5)