FastQCFastQC Report
Sat 18 Mar 2023
SRR6792044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123381369
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2653244621.504418547990014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27694952.2446622390776034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10312510.8358239241128862No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA10280730.8332481705564476No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA4631230.37535894094350664No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3638280.29488082596976206No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3298650.2673539795137141No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2903170.2353005176980975No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2612340.21172888752758126Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2090060.16939834733070597No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC2021160.16381403581281384Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1979490.1604367025624428No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1942010.15739896677593193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC1833280.14858645311351668No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1831610.14845110042505688No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1786790.14481846120543532No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1716310.1391060914553477No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1679210.136099154484175No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1590220.12888655822906292No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1555340.12605955117907633No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1531870.1241573190843749No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1521930.12335168691473995No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1518890.12310529639203469No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1352110.10958785843914572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG1349560.10938118217832385No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1303330.10563426314389493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1295600.10500775040030558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACCGCAG1265520.10256978101774832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGG1296300.070.25887145
TAGTACC469300.064.427145
AAGCAGT163258800.050.9993631
GTATCAA165371900.050.3684049
AGTGGTA164988250.050.3603825
AGCAGTG166801550.049.8248442
CAGTGGT169049000.049.1585164
GGTATCA169197600.049.143758
GTGGTAT169257350.049.0836526
TGGTATC170162300.048.853477
GCAGTGG171497400.048.3947833
CAGGGGG1365200.038.35692145
CAGGGGA2811400.033.38323145
CGTGGGA4202300.030.110346145
CATCGGG231900.027.608475145
CATGGGG15763850.027.342329145
CCTGGGG3372550.027.110601145
CATGCGG265200.026.903208145
TGCGGAT174600.026.328583145
CACGGGG1569450.025.903923145
Produced by FastQC (version 0.11.5)