FastQCFastQC Report
Sat 18 Mar 2023
SRR6792039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161501236
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG78653634.870156535520261No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG56069153.471747423654392No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA21582861.3363897722739408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGTGAGATGGCTCAGT19601601.2137120733862372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17614571.090677101691036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC12059240.7466964525274593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9068690.5615244950818828No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG6128090.3794453932228729No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTC5383320.3333299566821891No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGAGAGATGGCTCA3573800.22128623213756704No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGCGAGATGGCTCAG2128420.13178970345465343No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2098480.12993584767363636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGTGAGATGGCTCAGC1759390.10893972353251835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC1772150.0103.22646145
AAGCAGT88633850.087.822021
AGTGGTA89653450.086.649685
GTATCAA90150100.086.4167259
AGCAGTG90509450.085.816312
GTGGTAT90527500.085.792246
GGTATCA90781950.085.619938
TGGTATC90941800.085.406517
GCAGTGG91726900.084.599233
CAGTGGT92268900.084.207654
CCTCTTC7176500.067.64708145
CCCTCTT13362200.067.480385145
TGACTGG1386300.034.8227542
GTGACTG1482400.032.7216151
GACTGGA1561650.031.0055733
CGTGGGG604100.030.09945145
CAGGGGA2010200.029.004454145
AGTTCAG1689650.028.5960739
ACCCGAC11792300.026.5301360-64
ATGGGGG20021900.026.4077125-29
Produced by FastQC (version 0.11.5)