FastQCFastQC Report
Sat 18 Mar 2023
SRR6792035_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792035_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79386890
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5723920.7210157747708721No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGAT3315280.41761051478399014No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2872610.36184941871384557No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATAC1424030.17937848428122075No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGCTGAT1101820.13879117824114284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC2299350.0141.43341
AACATCG3705250.0141.212662
TGGAACA3897300.0140.927551
ATGAATC1956750.0140.159642
ACGCTTA1315200.0139.315252
ATAGCGA1046750.0139.164261
GCTGATC2398250.0139.073212
AGAGATC2241000.0138.486762
CCGACAA4150250.0138.383291
CAGATTC1737800.0138.080932
ACATCGA1225000.0137.491783
GGAACAA3965800.0137.479252
CCTAATC1824800.0137.369891
TCACGCA2031500.0137.194122
TAGCGAC1063150.0137.113892
CGACAAC4200300.0136.808122
ATCATTC2655400.0136.110031
GACAACT1455750.0135.817933
CTAATCC1841900.0135.816352
ACATCGG1250100.0135.212653
Produced by FastQC (version 0.11.5)