FastQCFastQC Report
Sat 18 Mar 2023
SRR6792034_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792034_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133619142
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC3956050.0141.577291
CAAGCTA4094050.0139.424332
ATGCCTA1897550.0138.270891
AGTTAGC2061850.0137.281252
ACAAGCT4288800.0136.931021
ACGTGAT2500250.0136.773152
GTCACTA1434950.0135.915332
GATCGCA2863550.0135.904462
GGCTAAC2252750.0135.758222
TGCCTAA1893350.0134.612562
CGTATCA4089900.0133.7732
AGTCACT1492550.0133.567551
ACGCTTA1246800.0133.22552
ATCGCAT1559900.0132.905873
AACGTGA2571400.0132.197421
AGATCGC3029750.0132.14641
AACATCG2032850.0131.404372
AGGCTAA2380750.0131.112411
GTGGTCA2245800.0130.992632
TCACGCA1671400.0130.52332
Produced by FastQC (version 0.11.5)