FastQCFastQC Report
Mon 27 Feb 2023
SRR3128955.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128955.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184560
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247991.1351942725308528No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG23620.10812245944263375No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC22120.1012560881825173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106350.084.962741
ACGGGTA2300.075.8824544
ATAGGGC13050.074.098743
ATAGCGG4550.071.554171
GAATAGG13600.071.470291
TAGTAGG8500.069.943731
GTAGGGC8600.069.658283
TAGGGTA4600.068.7043844
AGTAGGG34750.068.413732
AGATAGG14650.068.2803041
AATAGGG33600.068.08792
TAGGGCA9300.067.965634
GGTAAGG14800.067.588281
ATAGGGT7550.067.475213
TAGAGGG39050.067.040832
ATAGAGG15800.066.894151
AGGGATG31300.066.8112955
GATAGGG34150.066.4388052
TAGGGAT12500.066.0382464
AAGAGGG85150.065.9219062
Produced by FastQC (version 0.11.5)