FastQCFastQC Report
Mon 27 Feb 2023
SRR3128948.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128948.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518290
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304931.210861338447915No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG33840.13437689860977092No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC30990.123059695269409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT120700.086.174991
ATAGGGC12800.074.168323
AGGGATG37550.073.3440255
ATAGAGG18100.071.994771
GTACGGG6150.071.105062
GGTAAGG15450.070.9458851
TAGGGTC6500.070.858154
ATAGGGA19750.070.6750953
GAATAGG16500.070.13761
AATAGGG36150.069.328462
TAGGGCA12950.068.9542164
TAAGGGA18700.067.857713
TAAGAGG17250.067.360861
AGTAGGG39950.066.971212
AAGGGAT38300.066.631264
TAGAGGG47150.066.617362
AGGGTAC15200.066.1678855
GTAGGGT9450.066.144973
AAGAGGG93050.065.4404142
TATAGGG18050.065.3866652
Produced by FastQC (version 0.11.5)