FastQCFastQC Report
Mon 27 Feb 2023
SRR3128938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2562669
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT467871.8257137382939426No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG55080.2149321664249265No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT43070.1680669645592154No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT42610.1662719609906703No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC41920.16357945563785256No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCT38110.14871214347229392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT179400.087.401781
CGTAGGG7700.071.4048542
AGGGATG37250.070.1411065
TAGGGTA7800.069.88834
ATAGGGC12950.069.6742863
GGTAAGG14850.068.428131
TAGGGCG5500.068.35474
ATAGGGA20950.067.967253
GAGGGAT29350.067.888914
TATAGGG18350.067.8644562
AGTAGGG41200.067.5238342
ATAAGGG34350.066.898242
ATGAGGG41800.066.6670152
TAGAGGG45750.066.66322
GTAGGGC11900.066.344263
ATAGAGG16700.065.918421
AAGGGGC80300.065.604124
AAGGGTA13500.065.0951844
AGAGGGC37800.064.771953
AAGAGGG86150.064.530062
Produced by FastQC (version 0.11.5)