FastQCFastQC Report
Mon 27 Feb 2023
SRR3128914.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128914.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559581
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196720.7685632921950897No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG28230.11029148911481997No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC28130.10990080017002782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84050.080.680761
GAATAGG15600.074.510261
ATAGGGC13050.072.754233
GGTAAGG12700.071.1444851
ATAGCGG6550.071.127521
ACGGGTA2050.071.0735864
TAGACGG3550.070.257271
AGGGATG40400.069.6792455
AGTAGGG42750.068.606592
AGGGCAT19700.068.2272345
TAGGGCA11100.068.171574
ATAGGGA24250.067.644273
AAGAGGG93900.067.574922
TATAGGG19100.067.427112
TAGGGAT17450.067.335634
ATAGAGG17700.067.26531
AGGGAAT23800.067.136695
TAGAGGG45400.067.0867162
CGTAGGG7000.065.8029562
AAGGGCA38200.065.578914
Produced by FastQC (version 0.11.5)