FastQCFastQC Report
Mon 27 Feb 2023
SRR3128883.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128883.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974161
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149070.75510558662642No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28440.14406119865603667No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG27800.14081931514197676No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC27060.13707088732884504No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG22730.11513751917903352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53100.078.571751
ATAGGGC10850.075.216863
AGGGATG41450.073.690675
TAGGGCA9750.071.1232454
AGTACGG3000.070.794571
GTACGGG4350.070.489262
AGGGCAT16800.070.487965
ACGGGTA1750.070.0864644
CGTAGGG6100.069.600362
GTAGGGA15150.069.437023
ATAGAGG11600.069.16711
ATAGGGA20250.068.7223
TAGGGAT16700.068.641794
AGGGAAT20500.068.310285
AAGGGAT33550.068.19434
TAAGGGA15400.067.697153
CTACGAC7500.067.6684345
GTAGGGT6500.067.49453
GAATAGG11550.067.423391
ACGGGAT6300.067.390834
Produced by FastQC (version 0.11.5)