FastQCFastQC Report
Mon 27 Feb 2023
SRR3128882.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128882.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028183
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143360.706839570196575No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29740.1466337110605897No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC28940.14268929381618917No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG28030.13820251920068355No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG24050.11857904340979092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55600.079.7542951
CGTAGGG6750.079.439412
AGGGATG35450.073.447165
TAGGGCA10450.071.9594
ATAGGGC12950.070.769523
TAGAGGG35150.069.9860152
AGGGCAT18450.068.777895
GTACGGG4150.068.004562
ATAGGGA20050.067.9774863
GAATAGG11650.067.883051
AGGGAAT20600.067.303295
ATAGAGG12950.067.2480851
TAGGGAT15800.067.225314
AAGAGGG78500.067.169312
GGGCGAT11550.065.9195866
AGTAGGG31250.065.625312
CGAAGGG21750.064.661662
TAAGAGG16700.064.268231
AATAGGG33550.064.070572
ATGAGGG36200.064.057982
Produced by FastQC (version 0.11.5)