FastQCFastQC Report
Mon 27 Feb 2023
SRR3128878.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128878.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963672
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228420.7707330635778858No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC43780.14772215008948358TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG42180.14232344200032931TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG33560.11323790217001072TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86850.081.252561
ATAGGGA22950.074.130053
AGGGATG46800.073.80665
GAATAGG16500.072.421141
CGTAGGG8200.072.2146152
ATAGGGC14100.071.6618043
TAGGGTC7850.071.242374
GGATAGG17950.070.502321
GGTAAGG15700.070.418021
AGTAGGG47800.069.7086642
ATAGGGT10300.069.354523
ACGGGTA4300.068.854634
TAGAGGG54050.068.6909942
AGATAGG16500.068.429431
AAGAGGG105200.067.9488142
AATAGGG43750.067.5679862
GAGGGAT37700.067.439914
AGTAAGG18100.066.7990341
ATGTAGG8950.066.756921
TAGGGAT18750.066.6716844
Produced by FastQC (version 0.11.5)