FastQCFastQC Report
Mon 27 Feb 2023
SRR3128874.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128874.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906645
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220850.7598107095981793No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG40970.1409528855432982Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC38010.13076932339518585TruSeq Adapter, Index 23 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG30470.10482876305844023Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97550.079.736591
AGGGATG44750.073.088465
AGATAGG17200.072.125951
AGTAGGG44700.071.525192
TAGGGCA12750.071.381224
ATAGGGA28500.071.147633
AAGGGTA21900.070.8416444
AAGGGAT50150.070.80454
ATAGGGC17450.070.53133
TAGACGG3350.070.4027251
CGTAGGG7050.070.232322
GGTAAGG17500.069.811351
TAGAGGG52950.069.553972
GTAGGGC12600.069.236973
TAAGGGA24800.069.0226753
AGGGTAC13350.068.993385
AGTAAGG19750.068.5455251
AGGGAAT29150.068.353375
ACGGGTA4150.068.177344
ATAGAGG20100.067.8213041
Produced by FastQC (version 0.11.5)