FastQCFastQC Report
Mon 27 Feb 2023
SRR3128867.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128867.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166291
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409451.8900969445009927No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG48670.22466972350436762No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT39010.18007737649281652No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC38060.17569200075151492No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT32950.15210329544830312No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT29220.13488492543245575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT159450.086.896881
ATAGGGC13950.073.107933
AGGGATG36750.072.766915
GTACGGG4900.071.028972
ACGGGTA2000.070.496944
ATAGAGG16500.070.168481
ATAACGG1750.069.923991
GAATAGG13600.069.904211
AGTAGGG34900.068.4602052
AAGGGTA15250.068.4166954
TAGAGGG40100.068.261922
AAGGGAT34200.068.023354
TAGGGCA11400.067.61114
AGATAGG12750.067.551011
AGTACGG2100.067.2346041
AGGGCAT16300.066.604475
AGAGGGC31100.066.190053
ATAGGGA18500.065.797143
AGTAAGG14100.065.756391
GATAGGG32850.065.716912
Produced by FastQC (version 0.11.5)