FastQCFastQC Report
Mon 27 Feb 2023
SRR3128863.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128863.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693238
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56770.8189106771411838No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG42530.6134978174883662No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC40250.5806086798473251TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG38230.5514700579021923No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30790.4441476087577427No Hit
AAAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT14910.21507765009996568No Hit
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT13410.1934400595466492No Hit
TAGACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT11350.16372443518676125No Hit
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG10710.15449239655067956No Hit
AGAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT9980.14396210248139887No Hit
GAACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT9800.14136559161500092No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT9770.1409328398039346No Hit
GGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT8810.12708478184981206No Hit
GAAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT7870.11352522510306706No Hit
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT7850.1132367238956895No Hit
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG7690.11092871423666907No Hit
GGAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT7120.10270642982640882No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC6990.10083117197845473No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG900.093.993512
AGGGTCG800.088.118915
TAGCGGG3500.087.2796862
ATAGCGG1500.084.716371
CGGGAAT1500.084.5941545
CGTTTTT21300.083.96511
CGCTACG5400.083.549783
AGGGATG23050.082.983425
GTAGCGG800.082.363141
AGTACGG401.024091E-982.363141
AGGGCAT5500.082.03075
TAGGGCA3500.080.5658654
TCCGCTA5850.080.452391
AGGGTAC3450.080.371265
AAGGGTA5450.080.19634
GCTACGA5650.079.852894
ATGGCAC15800.079.716029
GATGGCA15900.079.510238
CTACGAC5700.079.152435
GGGCGAA950.079.152436
Produced by FastQC (version 0.11.5)