FastQCFastQC Report
Mon 27 Feb 2023
SRR3128838.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128838.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146402
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339251.5805520121580208No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG34990.16301699308889947TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC29240.13622797593367877TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT125550.084.6062551
ATAGGGC13150.077.067143
TAGGGCA10900.073.51554
AGGGATG36600.071.260885
AGATAGG12500.070.155371
TAGAGGG36950.068.631632
ATAGGGA18850.068.516593
AGGGAAT21250.068.1131745
GAATAGG13300.068.0624541
TATAGGG16500.067.133672
ATAAGGG30200.067.114762
GAGGGAT26850.066.5351944
GTAGGGA15600.066.1722643
AAGAGGG79800.066.038232
AGGGCAT16900.065.624295
TAGGGTA6850.065.3716664
AATAGGG30950.065.0314942
ATAGAGG13350.064.9822161
AGAGGGA49500.064.94353
AGTAAGG12550.064.6163941
Produced by FastQC (version 0.11.5)