FastQCFastQC Report
Mon 27 Feb 2023
SRR3128821.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128821.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917029
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115610.6030686025094039No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC41050.21413343251458378No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG31430.1639516147121405No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG29310.1528928357369659No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50550.076.80381
CGTAGGG5750.075.1954962
AGGGATG25450.072.756035
TAGGGTA5750.072.743464
ATAGCGG3500.072.606521
ATAGGGA14700.071.2950443
AGGGCAT16050.070.859955
ATAAGGG26400.070.67382
GTAGGGT6750.070.321713
GAATAGG13550.069.113631
GGTAAGG9800.068.668871
CGAAGGG23600.068.305232
AGGGTAC8900.068.117745
TAGACGG2300.067.520561
TAAGGGA17050.067.2569663
AAGGGAT32200.066.8469244
ACGGGAT6000.066.5793464
ATAGGGC9750.066.519093
TAGAGGG34750.066.4046552
AGTACGG2700.066.232291
Produced by FastQC (version 0.11.5)