FastQCFastQC Report
Mon 27 Feb 2023
SRR3128813.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128813.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367693
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229740.9703116071213624No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG27990.11821633970282464No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC25000.10558801331084731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103250.081.8666841
CGTAGGG6450.075.113942
GAATAGG15750.070.222811
AGGGATG35500.070.03315
TAGAGGG38200.069.324712
TATAGGG18050.069.318232
AGTAGGG38400.068.841152
GTAGGGC11350.068.7366263
TAGGGCA13050.066.6254
AGAGGGC36400.066.623023
ATAGAGG16300.066.120911
ATAGGGA21400.066.104143
AAGAGGG86650.065.141192
ATAGGGC13350.064.7757643
ATGAGGG39400.064.467462
GAGGGAT33250.064.312554
GTAGGGA19050.064.1437453
TAGGGAT15350.063.9902654
ATAAGGG29700.063.983492
AAGGGAT39100.063.945634
Produced by FastQC (version 0.11.5)