FastQCFastQC Report
Mon 27 Feb 2023
SRR3128746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173183
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206860.9518756588837665No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC30820.14181962586675856No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG25250.11618901859622498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81000.083.296521
AGGGATG37650.074.8943565
GAATAGG13900.074.129661
ATAGGGC12900.072.503023
AGGGTAC10350.071.2889255
GGTAAGG15950.071.091731
TAGGGCA8900.070.759964
ATAGAGG16000.070.57551
AGTAGGG36850.070.5294342
AGATAGG12350.070.099281
TAGGGAT14250.069.918774
TAAGAGG16150.068.754671
ATAGGGA19450.068.626443
GTAGGGA16600.067.384753
TAGAGGG40950.067.370062
AAGGGTA14600.067.276944
TAGGGTA6150.067.2481464
AAGGGAC21050.066.979514
ATGAGGG38200.066.560522
GTAGGGT6850.066.553963
Produced by FastQC (version 0.11.5)