FastQCFastQC Report
Mon 27 Feb 2023
SRR3128738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214846
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158380.7150835769168602No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG30440.1374361919519461No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC30360.1370749930243457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79600.081.9229661
TAGGGCA9900.073.107224
AGTAGGG37600.072.2869642
AGGGTAC12150.071.559995
ATAGGGC11850.071.388613
GAATAGG12050.069.500591
GTAGGGA16700.069.229863
CGTAGGG6050.067.621352
AGGGATG35400.067.0444645
ATAGAGG14400.066.980261
AAGGGAC22900.066.2890554
AAGGGAT35750.066.125164
ATAAGGG31450.065.639282
AGGGAAT22150.065.563115
TAGGGAT14000.065.460814
GTAGGGC10600.065.175793
ATAGGGA19400.065.166643
CGAAGGG25600.065.025442
AAGGGGC71950.064.927764
GGTAAGG13700.064.9077761
Produced by FastQC (version 0.11.5)