FastQCFastQC Report
Mon 27 Feb 2023
SRR3128735.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128735.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467454
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79720.5432538260143078No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC28970.19741675037173229No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28110.19155626002586792No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG24710.16838687958872986No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG23140.1576880774456985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41900.083.085741
ACGGGTA3000.079.896614
AGGGATG35300.079.88335
CGTAGGG3700.078.777572
GACCGTA255.5610602E-575.1968158
GAGGGAT26850.072.991324
TAGAGGG27050.072.473862
AGGGTAC7850.071.8440865
AAGGGAT34200.070.634434
CGAGGGA9200.070.497013
TAAGAGG11750.070.066271
ATAGAGG9950.069.975731
AGAGGGA39100.069.956113
AGAGGGC18650.069.804013
AGGGTAG9300.069.7389765
AGTAAGG10000.069.625851
GATGACC19850.069.609148
TAAGGGA15800.069.6046453
GTAGGGC5750.069.475313
AAGGGTA12150.069.239864
Produced by FastQC (version 0.11.5)