FastQCFastQC Report
Mon 27 Feb 2023
SRR3128638.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128638.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504605
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205430.8202091747002023No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG42970.17156397915040494Illumina PCR Primer Index 5 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG38940.15547361759638745Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC36500.1457315624619451Illumina PCR Primer Index 5 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28810.11502811820626405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71150.080.697851
ATAGCGG6100.073.29441
AGGGATG38050.071.637935
ATAGGGC15000.071.435383
TAGCGGG13050.070.9866942
GGTAAGG14050.070.00791
AGATAGG13000.069.870041
TAGGGTC7800.069.290384
AGTAGGG37950.068.522722
TAGAGGG44050.068.0009162
CGAAGGG24600.067.477782
ATAGGGA22850.067.461713
AGTACGG4400.067.385261
TAAACGG2450.067.232461
ATAAGGG28350.066.679792
GTAGGGA18550.066.378453
AAGGGAT47400.066.331154
AGGGCAT23150.066.181465
AAGGGCA38800.065.529254
AGGGTAC14650.065.4428565
Produced by FastQC (version 0.11.5)