FastQCFastQC Report
Mon 27 Feb 2023
SRR3128566.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128566.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516162
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223280.8873832447990233No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31370.12467400747646613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75200.082.841881
ATAGCGG6600.074.855751
GAATAGG17350.071.3240661
GTAGGGT9750.070.372223
ATAGGGC15900.070.049383
CGTAGGG6700.068.7678762
AGTAGGG43450.068.709732
GGTAAGG16050.068.306291
GGATAGG17000.067.810511
ATAGGGA24200.067.385613
AGGGATG41850.067.3765645
AGTAAGG17500.066.679671
ATGCGGG13050.066.649292
AAGAGGG86350.066.26172
GTAGGGC11950.065.6752243
TAAGGGA22900.065.670083
TAGGGCG4250.065.2403264
TAGGGCA13000.065.070214
AGGGAAT27200.064.445565
AGGGACT32000.064.177765
Produced by FastQC (version 0.11.5)