FastQCFastQC Report
Mon 27 Feb 2023
SRR3128534.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128534.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816817
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251920.8943427989819714No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG33580.11921257220472613TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG32110.11399391582768777TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC32040.11374540838116215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87600.082.943261
CGTAGGG7300.072.117282
ATAGGGC16050.070.863543
ATAGGGA29750.070.458023
AGTAGGG43100.068.0536652
AATAGGG41850.066.829122
TAGAGGG48350.066.4000552
AGGGCAT22750.066.314145
TAGGGAT18300.066.259964
GATAGGG39200.066.070822
GTAGGGA21900.065.454313
TAGGGCA14150.065.432324
AGGGATG39900.065.3736345
TATAGGG26050.065.319832
AAGAGGG97900.065.250072
TAAGGGA25950.065.2000053
TACGGGT2750.064.943173
ATAGCGG7250.064.908251
AAGGGTA17250.064.8440864
GAGGGAT34850.064.5974354
Produced by FastQC (version 0.11.5)