SRR3128453.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3128453.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1208762
Sequences flagged as poor quality	0
Sequence length	100
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9908	0.8196816246705306	No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	5659	0.46816494893122057	No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	4769	0.39453589705831255	RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	4476	0.37029622043049004	RNA PCR Primer, Index 41 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	4006	0.3314134626998532	Illumina PCR Primer Index 11 (95% over 24bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGACATC	3518	0.29104157807740483	No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA	1721	0.14237707671154454	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1575	0.13029860303351695	RNA PCR Primer, Index 41 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1248	0.10324613116560581	RNA PCR Primer, Index 41 (95% over 24bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG	1209	0.10001968956668061	No Hit

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	135	90.51681	4
ATAGCGG	230	87.95522	1
CGTTTTT	4775	86.89929	1
CGCTACG	980	85.36573	3
TCCGCTA	1040	84.13963	1
GCTACGA	1000	83.65842	4
AGGGATT	1880	81.99844	5
CGAAGGG	1090	81.06939	2
TAGCGGG	580	80.229256	2
TAGGGCG	170	80.17496	4
AGGGATG	2820	79.66517	5
CGAGGGA	680	79.48379	3
CGTAGGG	225	79.38284	2
GCGGGAT	750	78.95851	4
TAACGGG	310	78.84374	2
AAGGGAT	3160	78.82762	4
CTACGAC	1070	78.18544	5
GACCGAT	115	77.6507	8
TACGACC	1075	77.38458	6
CAGGGAT	1405	77.27255	4