FastQCFastQC Report
Mon 27 Feb 2023
SRR3128438.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128438.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578474
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164711.0434761674883464No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG40320.25543657988665003No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG38690.2451101506898435No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC34330.21748853639654503No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAGCTCCA32990.20899932466420099No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA16150.10231400707265371No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16090.10193389311448907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54600.083.144651
ATAACGG1200.074.485541
GAATAGG8800.073.238181
AGTAAGG9100.071.857661
ATAGGGA14000.071.169613
GTAGGGA10700.070.717823
TAGGGTA4400.070.498194
AGATAGG8050.070.126951
AGGGAAT16900.070.081055
GGTAAGG7200.069.911871
AATAGGG21000.069.840062
AAGGGTC7600.069.261384
CGTAGGG4550.069.220182
AGTTAGG4150.069.148281
ATAGAGG8950.068.3313451
TGTAGGG9700.068.3308262
TAGGGCA6750.068.235284
GAGGGAT17450.068.141524
TAGAGGG26700.067.958742
AGTAGGG22350.067.514462
Produced by FastQC (version 0.11.5)