FastQCFastQC Report
Mon 27 Feb 2023
SRR3128385.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128385.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213549
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207181.7072240181484226No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC64350.5302628900851963No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG61240.5046355771377999TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG48020.3956988963774845TruSeq Adapter, Index 14 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAGGCGTA37220.3067037260135355No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27210.22421838755583828No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT18640.1535990718133343No Hit
AGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT16140.1329983379327905No Hit
AGAGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT15000.12360440328326257No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA13630.11231520111672458No Hit
ACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG13570.11182078350359154No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG12970.10687660737226104No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG12590.10374529582241837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68400.087.951841
GAGGGAT11400.078.835084
ATAGCGG1300.076.040711
TAGGGCA6550.075.4291154
ACGGGTA1250.075.2854464
AGGGCAT11850.074.96375
TAGACGG2150.074.440741
AGGGATG12400.073.912945
ATAGAGG6950.073.826441
AGGGTAC6750.073.809065
AGGGTAT4600.073.5668645
ATAGGGC9900.072.7188953
AAGGGTA6650.072.1721344
AGTAAGG6900.070.268121
ATAGGGA9700.069.852483
ATAAGGG15300.069.5037162
TAGAGGG18500.069.435562
GTAGGGT4950.069.3918843
AAGGGAT16200.069.127844
TAAGGGT4500.069.011653
Produced by FastQC (version 0.11.5)