FastQCFastQC Report
Mon 27 Feb 2023
SRR3128315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852270
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114531.3438229669001607No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACG88051.0331233059945792No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG48890.5736445023290742No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC48180.5653138090041888No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC44450.5215483356213407No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG39530.46382015089114953No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATG27080.31773968343365366No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24790.2908702641181785No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGAATCACGTC18790.22047003883745758No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT15970.1873819329555188No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT15120.17740856770741667No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG14640.17177654968495898No Hit
AAAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT14320.16802187100332056No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGAA12690.1488964764687247No Hit
AGAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT11910.13974444718223097No Hit
GGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT10730.1258990695436892No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10590.1242563976204724No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT10400.12202705715324957No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGAATCACGTCGT9810.11510436833397866No Hit
GGAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT9250.10853368064111138No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC9170.10759501097070177No Hit
GGAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT8800.10325366374505732No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG201.828444E-594.094851
CGTTTTT47200.088.6126251
ACGGGTA800.088.1207664
ACGGGTC600.086.162524
AGGGATG18950.084.323125
AGGGTAG3200.082.246055
GTACGGG1600.082.246052
GGATGGC16050.081.6970147
GCTACGA10800.081.375714
TCCGCTA11100.080.955481
CGCTACG10950.080.690193
AGTACGG1350.080.154871
GAGGGTA4200.079.448564
GTAGGGC3200.079.308693
TACGGGT600.078.329573
CGTAGGG2100.078.329572
ATGGCAC14900.077.909019
GATGGCA15600.077.7270368
TACGACC11200.077.63026
AGGGCAT7150.077.56275
Produced by FastQC (version 0.11.5)