FastQCFastQC Report
Mon 27 Feb 2023
SRR3128311.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128311.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2762331
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379681.3744913263472045No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC65500.2371185784759321No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG60920.22053837863746234No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG52970.19175833743313167No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46370.16786547303708355No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33620.12170880318108149No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115550.085.717631
CGTAGGG6250.071.442712
TAGGGCA14850.071.214824
AGGGAAT25150.071.015335
AGGGATG42050.070.4175645
TAGGGTA9300.070.249984
GGTAAGG14950.070.152861
AGTAGGG44350.069.84032
ATAGGGC16100.069.1889653
ATAGGGA24200.069.143123
GTAGGGT11900.068.7252963
ATAGCGG5650.068.256991
TAAGGGA23700.067.8253563
AAGGGAT44550.067.7332154
AGGGCAT25900.067.507135
GAGGGAT36400.067.4036564
AGGGACT41700.067.28915
AGGGTAA13850.067.1926655
AAGAGGG91750.067.160042
TAGAGGG50700.067.118922
Produced by FastQC (version 0.11.5)