FastQCFastQC Report
Mon 27 Feb 2023
SRR3128071.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128071.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528833
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269741.066658019726886No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35670.14105320517408623No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34270.13551705470468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82100.085.528441
CGTAGGG7500.076.452532
ACGGGTA3800.074.209754
TAGGGTA10700.072.036634
ATAGAGG20500.071.351071
AGTAGGG42900.070.225372
TAAGGGA25850.068.362923
ATAGGGC15000.067.992623
TAGAGGG51200.067.928972
AGGGATG35800.067.7436455
ATAGGGT12550.066.660653
CGAAGGG28050.066.352242
GAGGGAT33450.065.616654
AAGAGGG87550.065.6006852
AGGGTAC12950.065.328795
ATAGCGG5650.064.929211
TAGCGGG13250.064.912522
AGATAGG13050.064.87181
GGGCGAT11900.064.378756
AGGGCAT22300.064.2831045
Produced by FastQC (version 0.11.5)