FastQCFastQC Report
Mon 27 Feb 2023
SRR3128070.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128070.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190822
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212320.9691339597648736No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT65190.2975595461429546No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG34810.15889013347501532No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG27750.12666478609398663No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC27200.1241543128560878No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22100.10087537919557135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59950.081.223651
ATAGGGC12050.075.66413
CGTAGGG6050.074.596752
GAATAGG14100.073.7402341
AGTAGGG39000.073.289722
TAGGGTA8450.071.7477344
GTACGGG4500.071.03962
AGGGATG29700.070.891925
ATAGGGA22300.070.812443
GTAGGGT11200.070.07673
TAAGGGA19000.069.754263
TAGGGCA10850.068.438824
GTAGGGA18550.068.4061363
AAGAGGG73250.068.1586462
ACGGGAT6100.067.7997364
ATAAGGG30850.067.202822
TAGGGTC6550.066.729344
AATAGGG31650.066.692462
GATAGGG30500.066.586782
TAGTAGG10400.066.499031
Produced by FastQC (version 0.11.5)