FastQCFastQC Report
Mon 27 Feb 2023
SRR3128058.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128058.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2747712
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235740.8579501781846133No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT61580.22411373535508816No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG50690.18448076072019193No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG41350.15048884308107982No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC39870.14510254349800852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69700.082.2681
AGGGATG42050.072.201645
TAGGGCG5800.072.117814
CGTAGGG7300.071.483722
TAGACGG4300.071.105791
ATAGGGA27600.070.667473
AGGGCGA15450.070.5732355
AGGGTAC13000.070.497195
TAGGGTA10700.070.277574
GAGGGAT38950.069.26044
GAATAGG18300.069.145071
AGGGTAT19000.069.013045
GTAGGGA26100.068.96663
AGTACGG3550.068.9025041
TAAGGGA25400.068.83193
TAGGGCA14450.068.627024
ATAGGGC18850.068.564913
AGGGAAT36600.068.0573965
AAGGGAC26100.067.8861854
AGTAGGG46400.067.68082
Produced by FastQC (version 0.11.5)