FastQCFastQC Report
Mon 27 Feb 2023
SRR3128057.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128057.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546987
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211360.8298432618619569No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG55930.21959279729342945No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT54710.2148028238856343No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG45520.1787209750187182No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC43200.16961217312848476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61200.082.13781
ATAGCGG4850.076.595311
AGGGATG35400.072.88655
GGTAAGG15500.072.507511
TAGACGG2400.072.494871
ATAGGGA25100.071.711043
TAGGGCA13250.071.6467364
GAATAGG16750.071.588321
TAAGGGA22700.070.804553
TAGGGCG4400.070.494014
TAGCGGG13350.069.349912
GAGGGAT33650.068.992654
AGTAGGG40650.068.673132
AGGGAAT32850.068.5295945
ACGGGAT8200.068.2015154
GTAAGGG39500.068.173962
ATAAGGG31300.068.1667252
TAGGGAT20900.068.1329654
ATAGGGC16800.067.9763643
TAAACGG4650.067.754581
Produced by FastQC (version 0.11.5)