FastQCFastQC Report
Mon 27 Feb 2023
SRR3128056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140820
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311050.9903464700301194No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT69630.2216936978241351No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG53830.1713883635483727No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG46600.14836889729433714No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC43490.13846702453499404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89700.081.671531
ATAGCGG5100.074.7290341
TAAGGGA30500.072.887253
AGGGATG45550.072.844995
ATAAGGG43100.071.643772
TAGGGCA16350.071.576744
ATAGGGC18850.071.30913
ATAGGGA29700.071.0526353
GAGGGAT44800.070.393864
TAGGGTA12350.069.64254
TAGACGG3400.069.193551
AGGGAAT39600.068.954775
AGTAGGG52500.068.663572
CGTAGGG7500.068.305472
AGGGTAC15200.068.024045
AGGGTAT18500.066.30595
TAGGGAT25900.066.234374
AAGGGAT59350.066.202724
AAGGGTA23000.066.00324
AAGGGCG14750.065.958174
Produced by FastQC (version 0.11.5)