FastQCFastQC Report
Mon 27 Feb 2023
SRR3128046.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128046.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195167
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117910.9865566904039351No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG53080.44412203482860557Illumina PCR Primer Index 5 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG47040.39358516424901285Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC44960.3761817386189545Illumina PCR Primer Index 5 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34110.2853994462698518No Hit
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT19300.1614837089712149Illumina PCR Primer Index 5 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT17620.1474270959623216No Hit
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT16800.14056613008893318Illumina PCR Primer Index 5 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT16460.13772133936094286Illumina PCR Primer Index 5 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG13190.11036114618291837Illumina PCR Primer Index 5 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT12700.10626130072199115Illumina PCR Primer Index 5 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT12470.10433688346482123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT36300.085.167171
TACGGGT1550.081.868783
CGGGAAT3500.080.5692755
GTAGGGC4500.079.3756563
AGGGATG26050.079.203265
CGAAGGG9550.077.287592
ACGGGTA2250.077.286834
AAGGGTA10300.077.114454
AACGGGT2500.077.0779343
CGTAGGG3050.077.0696952
CGCTACG7750.077.0172963
TAGGGCG1600.076.372964
GGAGCTA30750.076.267889
GGTAAGG7300.076.062861
AGGGTAC5650.075.697095
GCTACGA7950.075.670944
TAAGGGA12650.075.420913
ACGGGTC1250.075.197994
GAAGGGC14400.075.067443
AAGCGGG11950.074.7479252
Produced by FastQC (version 0.11.5)