FastQCFastQC Report
Mon 27 Feb 2023
SRR3128038.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128038.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626632
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290231.1049511313347284No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38280.14573796405434794No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG27890.10618160442726655RNA PCR Primer, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85900.083.694191
CGTAGGG7350.074.818452
ATAGGGC15250.072.1187063
GTAGGGA19450.069.352833
GAATAGG17950.069.199771
TAGACGG2450.069.1355741
TAGAGGG47250.068.8358152
ATAGAGG18400.068.785931
TATAGGG20300.068.302432
ATAGGGA24100.068.25793
AGTAGGG42250.068.0824052
TAAGAGG20800.067.8614351
AGAGGGC36000.067.628413
ATAAGGG38200.067.179922
AGGGATG41300.067.1422045
TAAGGGT11500.066.618013
TAGGGCA13100.066.3746644
TAGGGAT17350.066.369494
AAGGGTA16150.066.353564
GAGGGAT31550.065.547364
Produced by FastQC (version 0.11.5)