FastQCFastQC Report
Mon 27 Feb 2023
SRR3127998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062154
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117531.1065250425079602No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC30050.2829156600643598No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG26590.2503403461268328No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG26050.24525633759323034No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25640.2413962570399396No Hit
AGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT11370.10704662412418539No Hit
AAAGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT11350.10685832751182973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1850.086.378034
CGTTTTT36350.085.429161
AGTACGG1200.082.338831
AGGGATG25700.080.649485
CGTAGGG3050.080.1534652
AGGGTAC5550.079.603295
GGATGGC19550.077.411457
AGGGAAT17650.076.690915
AGGGCGA5850.076.3244865
AAGGGTA7800.075.922774
AGACGGG5950.075.853052
TAAGGGA11200.075.5354163
CGGGATG3550.075.4644855
ATGGCAC18700.075.14599
AACGAGG2950.074.962221
TACGACC4850.074.618266
GCATAGG3350.074.4385151
ACGGGTA1200.074.4163744
GAGGGAT15600.074.416374
AAGGGCG5250.074.304474
Produced by FastQC (version 0.11.5)