FastQCFastQC Report
Mon 27 Feb 2023
SRR3127993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3279602
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412491.2577440799218929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT123450.083.772111
CGTAGGG9400.075.499072
ATAGGGC20800.071.854883
GTAGGGC16100.071.2289353
AGGGATG52150.069.845735
TAGGGCA19350.069.709734
TAGAGGG60150.069.385682
AGTAGGG51300.068.346142
ATAGGGT15700.067.9545753
AGTAAGG21800.067.553951
AGAGGGC49600.066.9931263
TAGTAGG13900.066.682881
TAGGGTC11150.066.6000754
AGGGCAT28800.066.582515
ATAGCGG7000.066.542641
TAAGGGA32100.066.47273
ATAAGGG45050.066.2477952
GAGGGAT47150.065.789224
GTACGGG8300.065.6859362
ATAGAGG21600.065.565541
Produced by FastQC (version 0.11.5)