FastQCFastQC Report
Mon 27 Feb 2023
SRR3127986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2964919
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242670.818470926187191No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG52270.17629486674003572Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG42040.14179139463843698Illumina PCR Primer Index 7 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40830.13771033879846295No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC38010.1281991177499284TruSeq Adapter, Index 23 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34950.11787843108024199No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81500.080.883081
TAGGGCA15500.074.287074
CGTAGGG9200.073.583092
ATAGGGC18050.072.9053963
TAAGGGA26000.070.316153
AGGGAAT39000.069.532855
AGTAGGG44700.068.992152
ATAGCGG7300.068.9664841
ATAGGGA26600.068.906753
TAGCGGG14200.068.530732
TACGGGT1450.068.065983
ATAGAGG18550.067.7243041
AGGGCAT23550.066.655265
AGGGATG36700.066.463035
ATAAGGG43950.066.211762
TAGAGGG51500.065.998562
GAATAGG18550.065.948761
ATAACGG3750.065.24531
AAGAGGG98450.064.894392
TAGGGAT19650.064.81654
Produced by FastQC (version 0.11.5)