FastQCFastQC Report
Mon 27 Feb 2023
SRR3127972.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127972.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022358
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316881.0484528967117728No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34170.11305742072911283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97450.083.225541
TAGGGCA15900.073.013934
AGTAGGG50950.072.968962
ATAGGGC17450.072.723413
GAATAGG18100.071.995021
AGATAGG19100.071.427571
ATAGAGG23050.070.004261
TAAGGGA24750.069.884123
GGTAAGG19450.069.4166261
AAGGGTA25150.069.333294
ACGGGAT9300.069.237844
CGTAGGG8800.068.8992
TAGAGGG58000.068.718512
ATAGGGT14600.066.9601443
GTAGGGT14400.066.910963
AAGGGCG12600.066.398054
TAACGGG10300.066.1662752
ATGCGGG16250.065.945832
ATAGGGA30150.065.629733
AGTAAGG19700.065.43131
Produced by FastQC (version 0.11.5)