FastQCFastQC Report
Mon 27 Feb 2023
SRR3127959.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127959.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495870
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41570.8383245608728094No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG23320.4702845503861899No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC21680.4372113658821869No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG21360.4307580615887229No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT8260.16657591707503983No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT6890.138947708068647No Hit
TTAATGGGCGAGTCCTCCCTCCCAATTGGAAATATGAATCATCTACAGCC6770.13652771895859803No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT6620.13350273257103676No Hit
GGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT5900.11898279791074273No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG5880.11857946639240123No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5720.11535281424566923No Hit
GATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC5710.11515114848649848No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT5660.11414281969064471No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT5270.10627785508298546No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT5190.10466452900961946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT254.9189293E-793.993955
CGTTTTT17450.085.479031
ACGGGTA550.085.449054
CGAAGGG3750.082.756412
AGGGCGA3150.082.058215
AATGCGG750.081.560121
GCGAGTC1100.081.17668
TAGCGGG1050.080.606892
CGTAGGG1050.080.606892
GCGGGAT950.079.15284
TATCGGG301.4511133E-678.36782
ATAGGGC3000.078.328293
TACGGGT301.4554735E-678.328293
AGGGTAT2800.077.209315
GGGCGAT1850.076.211316
AGACGGG3950.076.185412
GGATAGG3000.075.286261
TTACGGG507.094059E-1175.2330862
TAGGGTA1500.075.195174
GCGATAT255.5580356E-575.195168
Produced by FastQC (version 0.11.5)