FastQCFastQC Report
Mon 27 Feb 2023
SRR3127950.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127950.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316275
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252181.0887308285933233No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28790.12429439509557372No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG28180.12166085633182588TruSeq Adapter, Index 23 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25580.1104359370109335No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG24840.10724115228114106TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC23700.10231945688659594TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78800.084.2673341
TAGGGCA12000.073.629774
TAGGGTA10100.071.194574
AGGGATG36600.070.881815
AGGGAAT23400.070.295745
ATAGGGA22450.069.920893
ATAGGGC13750.069.727533
AGGGTAC11050.068.9016345
ATAGCGG5000.068.708631
AGTAGGG38600.068.459242
AGATAGG13400.067.781461
AAGGGTA16100.067.723434
GAGGGAT29750.067.455564
ATGAGGG38300.067.39952
ATGCGGG11950.067.283932
TAAGGGA22750.067.13963
AGGGTAA15000.066.423455
ATAAGGG33700.066.274562
ATAGAGG16050.066.266171
ACGGGTA3200.066.0905464
Produced by FastQC (version 0.11.5)