FastQCFastQC Report
Mon 27 Feb 2023
SRR3127919.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127919.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2723786
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322251.183095881981918No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG36340.13341723615585072No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG36150.13271967768392964No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35210.12926859892810963No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC32230.11832794500008445No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30870.113334894885281No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95500.085.347331
TAGGGTA11100.076.213734
AGGGATG49750.075.669895
GAATAGG15500.074.03771
TAGGGCA14750.072.329854
ATAGGGC16850.071.683123
AGTAGGG47600.071.386322
AGGGTAC13250.071.295795
CGTAGGG8750.070.363422
AGTAAGG16250.070.331131
GAGGGAT35650.070.003334
TATAGGG23800.069.115392
GTAGGGA20300.068.529783
AGAGGGC43100.068.3713153
GTACGGG6050.068.3614042
AGGGTAG13300.068.200785
TAAGGGA23200.068.066743
AGGGAAT31950.067.8131265
ATAGGGA25550.067.508563
AAGAGGG92350.067.27882
Produced by FastQC (version 0.11.5)