FastQCFastQC Report
Mon 27 Feb 2023
SRR3127889.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127889.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372059
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266671.124213183567525No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG24550.10349658250490396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82400.086.3147051
AGTAGGG36800.071.771982
ATAGGGC14550.070.091143
GGGCGAT12250.069.823526
AGGGATG31450.069.785095
ATAGCGG5000.069.6180651
ATAACGG2600.068.749651
TAGTAGG11300.067.853051
ATAGGGA22950.066.757693
AAGAGGG77750.066.4903642
AAGGGAT36200.066.470324
AGGGCAT18550.066.124595
GTAGGGA18950.065.9688343
ATAAGGG36500.065.666472
TAGAGGG38950.065.517722
GAGGGAT30050.065.060184
AGGGAAT26950.065.045385
TAAGGGA22350.064.764883
TAGGGCA11400.064.723384
AGTAAGG12400.064.489271
Produced by FastQC (version 0.11.5)