FastQCFastQC Report
Mon 27 Feb 2023
SRR3127885.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127885.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3010284
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361631.2013152247429146No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46620.1548691086953922No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31710.10533889825677578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104150.083.3771061
AGGGATG56450.076.432465
GAGGGAT41700.071.344344
AGAGGGC43050.070.744673
TAGAGGG54650.070.349692
ATAGGGA30450.070.074573
CGTAGGG7650.070.039412
ATAGAGG22350.068.8247451
TAAGGGA26250.068.574293
AGGGAAT34050.067.6360245
AGTAGGG50900.067.499212
ATAAGGG44000.067.4024662
AGGGTAC14050.067.2386255
ATAACGG2450.067.2010961
GTAGGGA26600.066.965253
GAATAGG19200.066.151081
TATAGGG25900.066.0542452
AAGAGGG111200.065.512772
TAGGGAT26550.065.321144
TAAGAGG24000.065.073061
Produced by FastQC (version 0.11.5)