FastQCFastQC Report
Mon 27 Feb 2023
SRR3127880.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127880.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534897
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470621.856564586253406No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTT46760.18446508871958112No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT38360.15132764763223122No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT35610.14047908060958691No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG32360.1276580468555527No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC26610.10497467944456916TruSeq Adapter, Index 15 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26170.10323890872094607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT150000.088.118281
ATAGGGC15800.075.549773
AGTAGGG38500.070.920522
GAATAGG14850.069.6614761
TAGGGCA12850.069.487544
ATAGGGA19300.069.397533
AGGGATG33000.069.3540045
ACGGGAT5800.068.872744
ATGTAGG9350.068.3949051
GGTAAGG15300.067.61261
AATAGGG37750.067.4743652
ATAGAGG17600.067.3262251
AAGGGAT38950.067.2054144
ATAGCGG5900.066.9458541
AGTAAGG15600.066.6137851
GTAGGGT9250.066.047763
TAAGAGG19000.065.830091
AAGAGGG86800.065.295622
AGATAGG13900.065.288831
ATAAGGG35250.065.060462
Produced by FastQC (version 0.11.5)