FastQCFastQC Report
Mon 27 Feb 2023
SRR3127873.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127873.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779670
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93231.1957623096951273No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG38720.4966203650262291No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG35370.45365346877525103No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC33630.43133633460310133Illumina Single End Adapter 1 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21850.28024677106981155No Hit
AGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT12650.1622481306193646No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT10790.13839188374568728No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT10040.12877242936113997No Hit
ACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG9480.12158990342067799No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT9420.1208203470699142No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT9330.11966601254376852No Hit
GGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT8590.11017481755101519No Hit
ACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT7880.10106840073364372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT29100.088.724651
CGTAGGG1850.086.376082
AAGGGTA6400.082.24784
AGGGAAT20400.081.787045
TAGGGTA3450.081.736954
GGGAATG23850.081.385676
AGGGTAT4800.081.268675
ATAGCGG950.079.206591
AGGGTAG3950.078.529565
GACCGAT301.45593E-678.3312458
AAGGGCA9200.077.650114
CGAGGGA2850.077.506713
CGGGTAG850.077.4097065
TAAGAGG3600.077.075161
GGGCGAT2750.076.9070366
AGGGATG14150.076.7258765
GCGTAGG1050.076.1420441
CGCTACG4700.075.997983
CGGGTAT1250.075.1985
TAGGGTC1700.074.645074
Produced by FastQC (version 0.11.5)