FastQCFastQC Report
Mon 27 Feb 2023
SRR3127846.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127846.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109800
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT788833.738885202388852No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT51080.24210825670679686No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCT47190.22367049009384773No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT44600.2113944449710873No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG26480.125509526969381No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23480.11129016968433025No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC21980.1041804910418049No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT220200.089.817931
ATAGGGC12350.072.687863
AGGGATG28350.072.611635
ACGGGTA1900.071.736444
ATAGCGG3150.070.194811
AGTAGGG33250.068.8387152
TAGAGGG35200.068.7638242
ATAGGGA15950.067.479253
GTACGGG5300.067.395862
TAGGGCA8800.067.295084
GTAGGGA14300.066.719913
GAATAGG12900.066.37421
AGGGTAC9100.066.1079565
AAGGGTA12300.066.105324
GTTTTTT304200.065.277422
AGAGGGC27200.065.143023
AAGAGGG65900.065.043642
AGTAAGG13250.064.6209261
AGGGAAT20850.064.468145
CGTAGGG6350.064.393342
Produced by FastQC (version 0.11.5)