FastQCFastQC Report
Mon 27 Feb 2023
SRR3127843.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127843.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2600280
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352891.357123079053025No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41330.1589444213700063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118000.087.150521
CGTAGGG6200.079.60192
AGGGATG59550.076.398715
GAGGGAT52750.075.204854
GTACGGG5900.073.2928542
AAGGGTA23450.071.7572864
TAGGGCA16350.071.5825964
ATAGGGA30850.071.304433
TAAGGGA29050.070.868593
AGTAGGG44300.070.55762
ATAGGGC19100.070.381493
AAGGGAT68550.070.28174
ATAGCGG4050.069.694431
CGAAGGG24450.069.014672
TAGGGAT28700.068.784924
AGGGTAC18250.068.760795
TGTACGG2150.067.8305051
GAATAGG16250.067.742991
AGGGACT33200.067.667835
AGGGCAT21500.067.547995
Produced by FastQC (version 0.11.5)